Journal of Agricultural and Food Chemistry · 2008 · 36 citations · 13 references
AgricultureFood ScienceImmunologyAllergyFood AnalysisFood ChemistryAntigen Detection AssaysEnzyme-linked Immunosorbent AssayFood AllergensFood AllergyProtein AnalysisProteins In FoodsEnzyme-linked Immunosorbent Assay (Elisa)Food Allergy DiagnosticsImmunoassays For Food AllergensMajor Food AllergensNutsSandwich Enzyme-linked Immunosorbent AssayTree Nut Allergen IdentificationWalnut Allergy
Among food allergens of tree nuts, walnuts are a frequent cause of adverse food reactions in allergic patients. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of walnut soluble proteins in processed foods was developed. The sandwich ELISA is highly specific for walnut soluble proteins. The recovery ranged from 83.4 to 123%, whereas the intra- and interassay coefficients of variation were less than 8.8 and 7.2%, respectively. This study showed that the proposed method is a reliable tool for detection in the presence of hidden walnut proteins in processed foods.
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