SERUM LIPIDE ANALYSIS BY CHROMATOGRAPHY AND INFRARED SPECTROPHOTOMETRY

Abstract

A tentative method has been developed for the analysis of serum lipides, using chromatography and'infrared spectrophotometry.The extracted lipides are separated into three fractions by successive elutions from a silicic acid-celite column with chloroform-hexane (1: 19), chloroform, and methanol.By suitable infrared absQrption measurements of these fractions (redissolved in carbon disulfide), the amounts of cholesteryl esters, glycerides, total phosphatides, cholesterol, and free fatty acids can be estimated.The accuracy for a given component is somewhat dependent on the composition of the sample.In general the probable error is about lO% for the major components, slightly greater for unesterified cholesterol and fatty acid$.