IDENTIFICATION AND COMPARISON OF BIOMOLECULES IN MEDICINAL PLANTS OF TEPHROSIA TINCTORIA AND ATYLOSIA ALBICANS BY USING FTIR

Abstract

The aim of this study is to adopt the approach of metabolic fingerprinting through the use of Fourier Transform Infrared technique to understand the composition, chemical structure and discrimination of biomolecules in medicinal plants of Tephrosia tinctoria and Atylosia albicans. IR spectrum in mid infrared region (4000-400cm -1 ) was used for discriminating and indentifying various functional groups present in two different species of medicinal plants belonging to the family Leguminosae. Presence of C=O, C-H, C=C and C-O, C-C, C-O were identified. These bonding structures are responsible for the presence of alkyl groups, methyl groups, alcohols, ethers, esters, carboxylic acid, anhydrides and deoxyribose. In the present study FTIR Spectroscopy was used as a sensitive and effective assay for the detection and comparison of biomolecules between the two species of medicinal plants. The results showed that Tephrosia tinctoria and Atylosia albicans are rich in phenolic compounds.