Mapping What Young Students Understand and Value Regarding Sustainable Development

Abstract

Studies of chromosomes of <i>Cyrtodactylus jarujini</i> Ulber, 1993 and <i>C. doisuthep</i> Kunya et al., 2014 to compare microsatellite and TTAGGG sequences by classical and molecular techniques were conducted in Thailand. Karyological typing from a conventional staining technique of <i>C. jarujini</i> and <i>C. doisuthep</i> showed diploid chromosome numbers of 40 and 34 while the Fundamental Numbers (NF) were 56 in both species. In addition, we created the chromosome formula of the chromosomes of <i>C. jarujini</i> showing that 2n (40) = L^sm ₁ + L^sm ₂ + L^t ₃ + M^m ₁ + M^t ₄ + S^m ₂ + S^a ₂ + S^t ₅ while that of <i>C. doisuthep</i> was 2n (34) = L^sm ₃ + L^m ₂ + L^t ₃ + M^m ₁ + M^t ₂ + S^m ₄ + S^a ₁ + S^t ₁. Ag-NOR staining revealed NOR-bearing chromosomes in chromosome pairs 13 and 14 in <i>C. jarujini</i>, and in chromosome pairs 9 and 13 in <i>C. doisuthep</i>. This molecular study used the FISH technique, as well as microsatellite probes including (A)₂₀, (TA)₁₅, (CGG)₁₀, (CGG)₁₀, (GAA)₁₀, (TA)₁₅ and TTAGGG repeats. The signals showed that the different patterns in each chromosome of the Gekkonids depended on probe types. TTAGGG repeats showed high distribution on centromere and telomere regions, while (A)₂₀, (TA)₁₅, (CGG)₁₀, (CGG)₁₀, (GAA)₁₀ and (TA)₁₅ bearing dispersed over the whole genomes including chromosomes and some had strong signals on only a pair of homologous chromosomes. These results suggest that the genetic linkages have been highly differentiated between the two species.