Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

Shota Atsumi, Taizo Hanai, James C. Liao · Nature · 2008 · 1.9K citations

Biosynthesis, Engineering, Biochemistry +6

Identification of Mutations in Laboratory-Evolved Microbes from Next-Generation Sequencing Data Using breseq

Daniel E. Deatherage, Jeffrey E. Barrick · Methods in molecular biology · 2014 · 1.7K citations · Full text

Bioinformatics, Biology, Molecular Ecology +12

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

Yu Jiang, Biao Chen, Chunlan Duan et al. · Applied and Environmental Microbiology · 2015 · 1.3K citations · Full text

Lactic Acid Permeabilizes Gram-Negative Bacteria by Disrupting the Outer Membrane

Hanna‐Leena Alakomi, Eija Skyttä, Maria Saarela et al. · Applied and Environmental Microbiology · 2000 · 1K citations · Full text

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Mamie Z. Li, Stephen J. Elledge · Nature Methods · 2007 · 965 citations

Engineering, Genetics, Homologous Recombination +9