<i>In vitro</i> fecal fermentation profiles and microbiota responses of pulse cell wall polysaccharides: enterotype effect

Abstract

The gut microbiota community of individuals is predominated by diverse fiber-utilizing bacteria, and might have distinct fermentation outcomes for a given dietary substrate. In this research, we isolated pea cell walls (PCWs) from cotyledon seeds, and performed the <i>in vitro</i> fecal fermentation by individual <i>Prevotella</i>- and <i>Bacteroides</i>-enterotype inocula. The <i>Prevotella</i>-enterotype inoculum showed a higher fermentation rate and produced more short-chain fatty acids (SCFAs), especially propionate and butyrate, throughout the entire fermentation period from PCW degradation compared with the <i>Bacteroides</i>-enterotype one. Furthermore, the better monosaccharide utilization capacity of <i>Prevotella</i>-enterotype inoculum was shown, compared to the <i>Bacteroides</i>-enterotype inoculum. PCW fermentation with <i>Prevotella</i>- and <i>Bacteroides</i>-enterotype inocula resulted in different microbial changes, and the abundance of <i>Prevotella</i> and <i>Bacteroides</i> was promoted, respectively. These results may contribute to predicting the responses of <i>Prevotella</i> and <i>Bacteroides</i> enterotypes to diets and offer useful information in personalized nutrition.