Scientific Data · 2020 · 15 citations · 26 references
Life SciencesBiologyBotanyComparative GenomicsGenome AssemblyGenomicsDe Novo Genome AssemblyGenome SequencingMetagenome AssemblyMetagenomic AnnotationMolecular PhylogenyPhylogenomic AnalysisAssembly-based AnnotationBioactive Plant CompoundsFunctional AnnotationGenome ReconstructionHost-symbiont InteractionsPhylogenetic AnnotationSymbiont InteractionsTranscriptome Sequencing
Many animal phyla have no representatives within the catalog of whole metazoan genome sequences. This dataset fills in one gap in the genome knowledge of animal phyla with a draft genome of Bugula neritina (phylum Bryozoa). Interest in this species spans ecology and biomedical sciences because B. neritina is the natural source of bioactive compounds called bryostatins. Here we present a draft assembly of the B. neritina genome obtained from PacBio and Illumina HiSeq data, as well as genes and proteins predicted de novo and verified using transcriptome data, along with the functional annotation. These sequences will permit a better understanding of host-symbiont interactions at the genomic level, and also contribute additional phylogenomic markers to evaluate Lophophorate or Lophotrochozoa phylogenetic relationships. The effort also fits well with plans to ultimately sequence all orders of the Metazoa.
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BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
Felipe A. Simão, Robert M. Waterhouse, Panagiotis Ioannidis et al. · Bioinformatics · 2015 · 14.5K citations · Full text
QUAST: quality assessment tool for genome assemblies
Alexey Gurevich, Vladislav Saveliev, Nikolay Vyahhi et al. · Bioinformatics · 2013 · 11.5K citations · Full text