Fluorescent Janus emulsions for biosensing of <i>Listeria monocytogenes</i>

Abstract

Significance Listeria has been a strong foodborne pathogen for years and can lead to severe diseases. Efficient sensing methods for quick detections of Listeria are still in demand. Here we designed a biosensor that installed Listeria antibodies on the surface of hydrocarbon phase of Janus droplets. We also dissolved a pair of blocking and emissive dyes in the hydrocarbon and fluorocarbon phases of Janus droplets. With the existence of Listeria , Janus droplets will form agglutinations and tilt that could be easily tracked by fluorimeter. Our work provides a sensitive, simple, and quick method for the detection of Listeria .