Greatly enhancing the enantioselectivity of PvEH2, a Phaseolus vulgaris epoxide hydrolase, towards racemic 1,2-epoxyhexane via replacing its partial cap-loop

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T. Reetz, José Daniel Carballeira · Nature Protocols · 2007 · 866 citations

Life Sciences, Biotechnology, Molecular Biology +15

Fusion PCR and gene targeting in Aspergillus nidulans

Edyta Szewczyk, Tania Nayak, C. Elizabeth Oakley et al. · Nature Protocols · 2006 · 789 citations

Life Sciences, Genetics, Molecular Biology +13

Addressing the Numbers Problem in Directed Evolution

Manfred T. Reetz, Daniel Kahakeaw, Renate Lohmer · ChemBioChem · 2008 · 440 citations

Life Sciences, Biotechnology, Molecular Biology +16

Directed Evolution of an Enantioselective Epoxide Hydrolase: Uncovering the Source of Enantioselectivity at Each Evolutionary Stage

Manfred T. Reetz, Marco Bocola, Li-Wen Wang et al. · Journal of the American Chemical Society · 2009 · 151 citations · Full text

Life Sciences, Biochemistry, Chemistry +17

Discovery of Selective Menaquinone Biosynthesis Inhibitors against Mycobacterium tuberculosis

Joy Debnath, Shajila Siricilla, Bajoie Wan et al. · Journal of Medicinal Chemistry · 2012 · 127 citations

Pharmacy, Medicinal Chemistry, Pharmacology +17