Secondary metabolites of <i>Curvularia</i> sp. G6-32, an endophyte of <i>Sapindus saponaria</i>, with antioxidant and anticholinesterasic properties

Abstract

In the present study, the biological activity of an extract of the secondary metabolites (E-G6-32) produced by the Curvularia sp. G6-32 endophyte (isolated from the medicinal plant Sapindus saponaria L.) was investigated. The antioxidant potential was confirmed by the DPPH (22.5%) and ABTS (62.7%) assays, and the total phenolic compound content was 40 µg gallic acid equivalents/mg. The extract E-G6-32 displayed good inhibitory activity toward butyrylcholinesterase (BuChE; IC50 = 110 ± 0.05 µg mL−1). The extract E-G6-32 was subjected to spectroscopic and mass spectrometry analyses. Comparison with the literature data confirmed that (-)-asperpentyn (1) was a major component. Asperpentyn belongs to the epoxyquinone family, which has attractive structural complexity, diverse functional groups, and a broad range of biological activities, including specific enzyme inhibitory activity. Our results suggest that Curvularia sp. G6-32 is a promising source of bioactive secondary metabolites and contains (-)-asperpentyn, which has potential pharmaceutical interest.Secondary metabolites of Curvularia sp. G6-32, an endophyte of Sapindus saponaria, with antioxidant and anticholinesterasic propertiesAll authorsAndressa Domingos Polli , Marcos Alessandro dos Santos Ribeiro , Adriana Garcia, Julio Cesar Polonio , Caroline Menicoze Santos, Angela Aparecida Silva, Ravely Casarotti Orlandelli , Juliana Cristina Castro , Benício Alves Abreu-Filho , Marcia Regina Pereira Cabral , Maria Helena Sarragiotto, João Alencar Pamphile & João Lúcio Azevedo https://doi.org/10.1080/14786419.2020.1739681Published online:16 March 2020