Frontiers in Immunology · 2019 · 32 citations · 30 references
Life SciencesCell BiologyImmunologyCellular ImmunologyImmunoregulationMucosal ImmunologyGastrointestinal ImmunologyGut-associated Lymphoid TissueIntestinal Barrier FunctionMucosal Barrier FunctionMucosal ImmunityMucosal ToleranceEnteric InflammationGut Barrier ImmunityIntestinal Epithelial RenewalIntestinal Stem CellsIntestinal Tolerance MechanismsMhc Class Ii MoleculesMhc Class Ii TraffickingMucosal Tolerance Mechanisms
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.
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