Optimizing <i>de novo</i> genome assembly from PCR-amplified metagenomes

Abstract

PCR-amplified metagenomes have enabled scientists to explore communities traditionally challenging to describe, including some with extremely low biomass or from which DNA is particularly difficult to extract. Here we show that a modified assembly pipeline can lead to an improved <i>de novo</i> genome assembly from PCR-amplified datasets, and enables a better genome recovery from low input metagenomes.