Fungal Endophytes Control Fusarium graminearum and Reduce Trichothecenes and Zearalenone in Maize

Abstract

<i>Fusarium graminearum</i> can cause Giberella Ear Rot (GER) and seedling blight in maize, resulting in major yield losses. Besides GER, the infected grains are consequently contaminated with multiple mycotoxins of <i>F. graminearum</i>. Zearalenone and trichothecenes, such as deoxynivalenol and its acetylated forms, are among the major mycotoxins associated with <i>F. graminearum</i> infection in maize. In the current work, we explored the effect of the endophytic fungal genera of <i>Epicoccum</i> and <i>Sordaria</i>, to control <i>F. graminearum</i> infection in comparative trials with <i>Piriformospora</i> spp., an elusive endophytic genus. Furthermore, we investigated the effect of these endophytes on zearalenone, deoxynivalenol, and 15-acetyldeoxynivalenol levels using in vitro and in planta assays. As plants are endowed with several detoxification mechanisms comprising e.g., glucosylation of trichothecenes, the effect of the isolated fungal endophytes on the deoxynivalenol-3-glucoside level was also assessed. In general, results showed a considerable variability in the antifungal activity, both among species and among isolates within one species. Additionally, the effect on mycotoxin levels was variable, and not necessarily related to the antifungal activity except for zearalenone levels which were consistently reduced by the endophytes. These results highlight the great potential of certain endophytic fungal strains as new biocontrol agents in agricultural science.