Scientific Reports · 2018 · 44 citations · 38 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionProtein TranslationProtein-protein InteractionsRna-binding ProteinsTranslation FactorsTranslation In EukaryotesTranslation InitiationTranslation RegulationTranslational RegulationMrna Translation InitiationPoly(a)-binding ProteinsPoly(a)-mediated Translation ControlPoly(a)-tail-dependent TranslationProtein-rna Recognition MotifsRna MotifsRna Recognition MotifsRna-binding Proteins In Translation
Eukaryotic mRNA has a cap structure and a poly(A) tail at the 5' and 3' ends, respectively. The cap structure is recognized by eIF (eukaryotic translation initiation factor) 4 F, while the poly(A) tail is bound by poly(A)-binding protein (PABP). PABP has four RNA recognition motifs (RRM1-4), and RRM1-2 binds both the poly(A) tail and eIF4G component of eIF4F, resulting in enhancement of translation. Here, we show that PABP interacts with the 40S and 60S ribosomal subunits dynamically via RRM2-3 or RRM3-4. Using a reconstituted protein expression system, we demonstrate that wild-type PABP activates translation in a dose-dependent manner, while a PABP mutant that binds poly(A) RNA and eIF4G, but not the ribosome, fails to do so. From these results, functional significance of the interaction of PABP with the ribosome is discussed.
38
Structures of the human and Drosophila 80S ribosome
Andreas M. Anger, Jean‐Paul Armache, Otto Berninghausen et al. · Nature · 2013 · 649 citations · Full text
Structure of the human 80S ribosome
Heena Khatter, Alexander Myasnikov, S. Kundhavai Natchiar et al. · Nature · 2015 · 536 citations