AIChE Journal · 2018 · 29 citations · 17 references
PharmacyBiotechnologyGeneticsMetabolic EngineeringMetabolismNatural Product BiosynthesisBiosynthetic Gene ClustersMetabolic Pathway EngineeringMicrobial Metabolic EngineeringMicrobial Natural Product BiosynthesisNonribosomal Peptide BiosynthesisPolyketide BiosynthesisBiosynthetic Gene Cluster AnalysisBiosynthetic Pathway AnalysisBiosynthetic Pathway MiningClustered Biosynthetic PathwaysGenome MiningHeterologous Expression SystemsHeterologous Gene ExpressionHybrid Biosynthetic Gene Clusters
Significance A polyketide synthase and nonribosomal peptide synthetase hybrid gene cluster from the genome of Penicillium thymicola was identified through genome mining. Heterologous expression of this cluster leads to the production of pyranonigrin A. A series of experiments established that only four genes are sufficient to biosynthesize pyranonigrin A. Based on the results from the current study, a biosynthetic pathway of pyranonigrin A is proposed. © 2018 American Institute of Chemical Engineers AIChE J , 64: 4182–4186, 2018
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NCBI BLAST: a better web interface
Margaret E. Johnson, Irena Zaretskaya, Y. Raytselis et al. · Nucleic Acids Research · 2008 · 4.5K citations · Full text