Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with<i>p</i>-nitroaniline

Abstract

A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized<italic>p</italic>-nitroaniline reagent, and quantitation by image analysis using the ImageJ software.