Science Advances · 2018 · 262 citations · 67 references
EngineeringBiological EngineeringBiotechnologyMetabolic EngineeringNatural Product BiosynthesisSynthetic BiologyFungal BiotechnologyFungal Natural Product BiosynthesisMetabolic Pathway EngineeringMicrobial Natural Product BiosynthesisMicrobial Synthetic BiologySynthetic Pathway ConstructionFungal Gene ExpressionFungal Metabolite BiosynthesisHeterologous Expression SystemsHeterologous Gene ExpressionHeterologous Pathway ExpressionMetabolic Engineering Of FungiMicrobial Expression SystemsRecombinant Protein Expression In Fungi
. We applied this platform to 41 fungal biosynthetic gene clusters from diverse fungal species from around the world, 22 of which produced detectable compounds. These included novel compounds with unexpected biosynthetic origins, particularly from poorly studied species. This result establishes the HEx platform for rapid discovery of natural products from any fungal species, even those that are uncultivable, and opens the door to discovery of the next generation of natural products.
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