Scientific Reports · 2017 · 32 citations · 24 references
MedicineImmunologyOphthalmologyImmunopathologyInflammationInnate ImmunityCorneal ImmunologyCytokine BiologyImmunopathology Of The EyeInflammatory Skin DiseasesOcular Cytokine BiologyOcular InflammationCorneal ImmunopathologyCorneal Inflammatory ResponseCorneal Innate ImmunityCytokine Production By Mast CellsGroup 2 Innate Lymphoid CellsOcular Surface KeratoconjunctivitisSurface EpitheliumTransgenic Mouse Models
In a transgenic mouse line hK14mIL33tg, with the expression of interleukin-33 (IL-33) driven by a keratin 14 promoter, keratoconjunctivitis developed spontaneously between 18 and 22 weeks of age under specific-pathogen-free conditions. These mice showed blepharitis and corneal impairments, and the histology revealed epithelial thickening in the conjunctiva and the cornea with infiltration of eosinophils, mast cells and basophils. IL-5, IL-13 and CCL11 were abundant in lacrimal fluid in the mice, and the gene expressions of IL-4, IL-5, IL-13, IL-33, Prg2 and Mmcp8 were significantly increased in the cornea. Furthermore, group 2 innate lymphoid cells (ILC2) producing IL-5 and IL-13 were markedly increased in the cornea. These phenotypes closely resemble human atopic keratoconjunctivitis (AKC). The characteristic ocular phenotype in these mice strongly suggests that IL-33 is crucial for the development of AKC. The mouse line may be useful as a novel model for research and development of therapeutic strategies for AKC.
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Innate production of TH2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphoid cells
Kazuyo Moro, Taketo Yamada, M. Tanabe et al. · Nature · 2009 · 2K citations