CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli

In-gel digestion for mass spectrometric characterization of proteins and proteomes

Andrej Shevchenko, Henrik Tomas, J. Havli et al. · Nature Protocols · 2006 · 5K citations

Protein Chemistry, Proteomic Technology, Engineering +9

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

F. Ann Ran, Patrick D. Hsu, Chie-Yu Lin et al. · Cell · 2013 · 3.3K citations · Full text

Rna-guided Crispr Cas9, Genetics, Molecular Biology +7

Automated design of synthetic ribosome binding sites to control protein expression

Howard M. Salis, Ethan A. Mirsky, Christopher A. Voigt · Nature Biotechnology · 2009 · 1.9K citations · Full text

Engineering, Protein Expression, Protein Folding +14

Programming cells by multiplex genome engineering and accelerated evolution

Harris H. Wang, Farren J. Isaacs, Peter A. Carr et al. · Nature · 2009 · 1.6K citations

Engineering, Medicine, Computational Biology +10

JCat: a novel tool to adapt codon usage of a target gene to its potential expression host

Andreas Grote, Karsten Hiller, Maurice Scheer et al. · Nucleic Acids Research · 2005 · 1.5K citations · Full text

Genetics, Molecular Biology, Escherichia Coli +21