Organic & Biomolecular Chemistry · 2016 · 54 citations · 52 references
Life SciencesBiotechnologyMolecular BiologyMolecular BiotechnologyProtein EngineeringSynthetic BiologyLigand BindingNucleic Acid EngineeringOligonucleotide ChemistryAptamer DiscoveryAptamer Library ConstructionAptamer RecognitionAptamer SpecificityAptamersCell-selexNucleoside ModificationSelex
, 47.5%). When the same sequences are synthesized with TTP, no binding is observed. Taken together, these findings highlight the value of using modified nucleotide triphosphates in aptamer selections and portends success in SELEX against an array of whole cells as targets.
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Clustal W and Clustal X version 2.0
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In vitro selection of RNA molecules that bind specific ligands
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