Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique

Abstract

Mammalian artificial chromosomes (MACs) are appealing vectors for transgenesis and gene therapy applications. Their principal benefits include large DNA-carrying capacity and the ability to replicate in synchrony with the host genome. In addition, introducing genes into artificial chromosomes eliminates the disadvantages of integrating exogenous DNA into the host genome—variegated gene expression and insertional mutagenesis.