Antimicrobial activity of olive mill wastewater extract against Pseudomonas fluorescens isolated from mozzarella cheese

Abstract

Olive mill wastewater polyphenol extract was tested for antimicrobial activity against 64 strains of <i>Pseudomonas fluorescens</i> responsible for mozzarella discolouration. The extract showed a minimum inhibitory concentration (MIC)₅₀ value of 5 mg/mL and a MIC₉₀ value of 7 mg/mL. The MBC₅₀ and MBC₉₀ values corresponded to 6 and 8 mg/mL, respectively. The MIC concentration (7 mg/mL) was demonstrated to have a bacteriostatic effect while maintaining the bacterial concentration on the levels of the inoculum for 48 hours. The 3/2 MIC concentration was responsible for four logs CFU/mL depletion in colony count after 24 h. As the extract concentration decreased from MIC value, no inhibitory effects were recorded.