1987 · 20 citations · 0 references
AgricultureFood ScienceNutritional SciencesChromatographic AnalysisFood AnalysisFruit ScienceFruit BiochemistryFruit Juice ExtractionFruit NutritionPhenolic Compound AnalysisVitamin AnalysisVitamin ChemistryCitrus FruitsHigh-performance Liquid ChromatographyHigh-performance Liquid Chromatography ColumnsJuice Composition AnalysisVitamin C Status
Abstract: A reversed-phase, high-performance liquid chromatography (HPLC) method is reported for the determination of ascorbic acid in commercial Florida citrus juices for nutrition labeling that compares well with results obtained using the AOAC indophenol titration method (R = 0.98). The HPLC method uses a Zorbax ODS column eluted with a mobile phase of 2% KH2PO4 (pH 2.4) at 0.5 mL/minute. An HPLC chromatogram, recovery data, compositional results, and data on the stability of ascorbic acid in orange juice are included.(wz)