Separation and estimation of saturated C2–C8 fatty acids by buffered partition columns

Abstract

recently been shown (Peters & Wakelin, 1948; Grob, 1946) that trypsin and chymotrypsin are inactivated by thiol compounds, whereas the skin proteinase is not, the conclusion stands firm at present that it is a proteinase of different type.SUMMARY 1.The proteinase present in acetone-dried extracts and in Fruton-type extracts of fresh skin from the rat have been compared and further characterized in the light of Fruton's results.2. The acetone-dried extracts have high pro-teinase activityand low dermo-and amino-peptidase activity with only slight activation effects of man- ganese, whereas the fresh skin extracts have a high peptidase and low proteinase activity.3. The skin proteinase differs from,previously recognized enzymes.4. Manganese activates the leucine aminopeptidase optimally at 0-002M; cobalt and magnesium also activate.We are indebted to Prof. K. Linderstrom-Lang for his hospitality and kindness in showing his technique; also to