Colorimetric detection of Maize chlorotic mottle virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynapthol blue dye

Abstract

<italic>Maize chlorotic mottle virus</italic> causes corn lethal necrosis disease, and can be transmitted <italic>via</italic> infected maize seeds. A colorimetric assay for the detection of <italic>Maize chlorotic mottle virus</italic> was developed which utilises RT-LAMP and hydroxynapthol blue dye (HNB).