Anticancer Activity of Withania Somnifera (Leaves) Flavonoids Compound

Abstract

In this research article make known on Withania somnifera (Ashwagandha) as Medicinal plants have therapeutic potential due to the presence of natural antioxidants functioning as reducing agents, free radical scavengers and quenchers of singlet oxygen. And this article also determines the use of Withania Somnifera (leaves) Polyphenolic Compound activity on MCF-7, A549 and PA-1 cancer cell line (breast, lung and ovary respectively). By providing a scientific basis the study can be made conventional to evaluate its constituents (natural product) to determine which of Withania Somnifera (leaves), would facilitate further study as potential new anticancer agents or lead to new anticancer compounds. Hydro alcoholic (1:1) sample of Withania Somnifera (leaves) were prepared and tested for their cytotoxic activities against cancer cell lines (MCF7, A549and PA1) with standard Doxorubicin. The most essential reason of this study is to estimate cytotoxicity of certain important Indian medicinal plants with facilitate of MTT assay. Concentrations are set of each plant extract which are 100 µg/ml, 10 µg/ml , 0.1 µg/ml, 0.01 µg/ml and 5-10×10 3 cells/ml are taken into each well which are exposed to different Concentrations of Withania Somnifera (leaves) for 96 hr and then treated with MTT. For MTT absorbance in use at 570 nm. From IC50 values of MTT assay of Withania Somnifera (leaves) for MCF7, A549 and PA1 cancer cell lines, from this it may conclude that Withania Somnifera (leaves)shows efficient cytotoxicity on MCF-7 (10 ± 1 µg ) than PA-1 (13 ± 1 µg) and A-459 (11± 1 µg) cancer cell line.