GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF BIOACTIVE CONSTITUENTS IN THE ETHANOLIC EXTRACT OF SACCHARUM SPONTANEUM LINN.

Abstract

Objective: To isolate and analyze the chemical composition in Ethanolic extract from the whole plant of Saccharum spontaneum Linn. by gas chromatography-mass spectrometry(GC-MS). Methods: The shade dried whole plant powder was extracted with ethanol by using Soxhlet extractor and crude ethanolic extract was obtained. Results: Qualitative analyses of ethanolic crude extract of S. spontaneum by using GC-MS showed that they were different types of high and low molecular weight compounds. Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important. Further, the Saccharum spontaneum possessed certain characteristics that can be ascribed to cultivation on a domestic plantation. The crude extract was prepared from the whole plant powder of S. spontaneum for respective compounds can be chosen on the basis of above GC-MS analysis. Conclusion: The bioactive compounds in the ethanolic extract of S. spontaneum have been screened using this analysis, Isolation of individual components would however, help to find new drugs for various ailments by traditional practitioners.