Methods · 2015 · 130 citations · 77 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene Regulatory NetworksSynthetic BiologyCell-free Synthetic BiologyGene SynthesisProtein SynthesisSynthetic Biology Circuit ModelingSynthetic Gene CircuitsSynthetic Gene NetworksCell-free Genetic CircuitsCell-free PrototypingCell-free Synthetic Gene NetworksCell-free Transcription-translation SystemsCell-free Translation Systems
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Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.9K citations
Engineering and characterization of a superfolder green fluorescent protein
J.D. Pédelacq, Stéphanie Cabantous, Timothy H. Tran et al. · Nature Biotechnology · 2005 · 2.5K citations
Cell-free translation reconstituted with purified components
Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari et al. · Nature Biotechnology · 2001 · 2K citations