Annual Review of Genetics · 1985 · 340 citations · 128 references
Life SciencesBiotechnologyMolecular BiologyDna ReplicationGenetic EngineeringSynthetic BiologyMutagenesis StrategiesNucleic Acid SynthesisOligonucleotide ChemistryPlasmid BiologyPlasmid ReplicationSite-directed MutagenesisBase Pairing PropertiesCassette MutagenesisHeteroduplex Dna FormationOligonucleotide SynthesisOligonucleotide-directed MutagenesisPoint MutationsSolid-phase Dna SynthesisSolid-phase Oligonucleotide Synthesis
TOTAL SYNTHESIS OF MUTANTS . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 Solid Phase Chemical Synthesis of Oligodeoxyribonucleotides .. . ..... 424 Assembly of Oligodeoxyribonucleotides into Double-Strand DNA 427 Mutation by Partial Gene Synthesis (Cassette Mutagenesis) 428 OLIGODEOXYRIBONUCLEOTIDE HETERODUPLEX MUTAGENESIS . . . . . . . . . . . . . . . 428 Oligonucleotide Mutagenesis Using Single-Strand Vectors 431 Oligonucleotide Mutagenesis Using Double-Strand Vectors ........ 433 Plasmids with a Filamentous Phage Origin of Replication 435 Selection and Enrichment of Mutants 435 Screening for Mutants 437 TARGETED RANDOM POINT MUTATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 438 Chemical Reagents that Change Base Pairing Specificity 439 Chemical Reagents that Eliminate Base Pairing. . . . ..... . 440 Enzymatic Incorporation of Nucleotide Analogs that Induce Mispairing ... 441 Enzymatic Misincorporation of Nucleotides.. 441 Random Point Mutations Using Long Synthetic Oligonucleotides with a Low Level of Misincorporuted Nucleotides ..... .... . ..... 443 Screening and Selection for Mutations Produced by Random Point Mutagenesis..... 444 DELETION AND INSERTION MUTAGENESIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 444 Deletion Mutagenesis .... .... . . 444 Linker Insertion Mutagenesis . . . . 445 IN VITRO CONSTRUCTIONS THAT TARGET IN VIVO MUTAGENESIS . . .. . . . . . . . . 446 ALLELE REPLACEMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446 CHARACTERIZATION OF MUTANTS 447 In Situ Characterization of Mutants .. .... . .. . . . . .. . .. . . . . . . . . . . . . . . . . . . . . . . . ...... . . . . . . .... . . 447 Recovery of Genetic Functions Prior to Characterization 448 Reversion by Site-Specific Mutagenesis 448
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Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA
Ellson Y. Chen, Peter H. Seeburg · DNA · 1985 · 2.5K citations
In vitro insertional mutagenesis with a selectable DNA fragment
Pierre Prentki, Henry Krisch · Gene · 1984 · 1.7K citations