Genes & Development · 2012 · 31 citations · 26 references
Life SciencesDevelopmental BiologyMolecular BiologyCell ProliferationGene ExpressionTranscriptional RegulationCell Cycle ControlCell Cycle Gene RegulationCell Cycle SignalingCell-cycle RegulationCyclin-dependent KinasesSmall Nuclear Rna BiologyCell Cycle Gene TranscriptionCyclin-dependent Kinase ComplexesDevelopmental Cell-cycle ControlGene-specific Transcription ControlP-tefb RegulationPrimordial Germ Cell ProliferationTranscription Elongation ComplexesTranscription Elongation Control
Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15(INK4b), a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.
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Blimp1 is a critical determinant of the germ cell lineage in mice
Yasuhide Ohinata, Bernhard Payer, Dónal O’Carroll et al. · Nature · 2005 · 1K citations