Isolation of Lactobacillus plantarum from cow milk and screening for the presence of sugar alcohol producing gene

Abstract

Lactobacillus plantarum, was isolated from cow milk, characterized by partial 16S rRNA gene sequencing and phenotypic features and resoluted for the presence of bacteriocin and their activity against pathogenic organism. The organism showed very strong activity againstStreptococcus faecalis and strong activity against Bacillus mycoides, Klebsiella pnuemoniae,Staphylococcus aureus and Proteus vulgaris. The organisms Psuedomonas aeruginosa andSalmonella typhi were resistant to the isolate. For the isolate, low calorie sugar alcohol, namely sorbitol, producing genes were screened and sequenced.   Key words: Lactobacillus plantarum, antimicrobial activity, sorbitol producing gene