Concepedia

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

Willem P.C. Stemmer, Andreas Crameri, Kim D. Ha, Thomas M. Brennan, Herbert L. Heyneker

Gene · 1995 · 731 citations · 18 references

DOI

Concepts

References

18

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

Francisco Bolívar, Rodriguez Rl, Greene Pj et al. · PubMed · 1992 · 4.7K citations

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Rapid evolution of a protein in vitro by DNA shuffling

Willem P.C. Stemmer · Nature · 1994 · 1.9K citations

, , +10

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

Willem PC Stemmer · Proceedings of the National Academy of Sciences · 1994 · 1.3K citations

, , +15

Randomization of genes by PCR mutagenesis.

R C Cadwell, Gerald F. Joyce · Genome Research · 1992 · 1.1K citations · Full text

, , +17

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

Wayne M. Barnes · Proceedings of the National Academy of Sciences · 1994 · 1K citations · Full text

, , +15