Effect of acid hydrolysis of Garcinia kola (bitter kola) pulp waste on the production of CM-cellulase and - glucosidase using Aspergillus niger

Caritas U. Okafoagu, Humphrey Chukwuemeka Nzelibe

AFRICAN JOURNAL OF BIOTECHNOLOGY · 2006 · 11 citations · 12 references

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Abstract

Effect of acid hydrolysis of bitter kola (Garcinia kola) pulp wastes on the production of CM-cellulase and -glucosidase using Aspergillus niger was investigated. Hydrolysis for 3 h with 2.5 M sulphuric acid yielded highest fermentable sugar. Acid hydrolysis enhanced CM-cellulase and -glucosidase levels by 500% at 96 h and 200% at 120 h, respectively. Acid hydrolysed G. kola pulp wastes is a better substrate for the production of CM-cellulase and -glucosidase compared with maize and rice husks

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