Substrate Specificity in Ketosynthase Domains from <i>trans‐</i>AT Polyketide Synthases

Abstract

Branching out: The substrate specificity profiles for a range of ketosynthase (KS) domains from trans-AT PKSs are reported. Evidence is provided that a sterically demanding amino acid residue adjacent to the active-site Cys residue confers specificity towards non-β-methyl-branched substrates (see scheme).