A drosophila protein that imparts directionality on a chromatin insulator is an enhancer of position-effect variegation

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila

Jay H. Chung, Mary Whiteley, Gary Felsenfeld · Cell · 1993 · 861 citations

Life Sciences, Genetics, Molecular Biology +4

tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.

Natalia Azpiazu, Manfred Frasch · Genes & Development · 1993 · 719 citations · Full text

Life Sciences, Developmental Biology, Genetics +14