Publication | Open Access
Enhancement of Recombinant Hemoglobin Production in <i>Escherichia coli</i> BL21(DE3) Containing the <i>Plesiomonas shigelloides</i> Heme Transport System
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Citations
14
References
2008
Year
EngineeringMolecular BiologyEscherichia ColiTransport GenesAnalytical UltracentrifugationHeme TraffickingBiochemical EngineeringE. Coli Bl21Heme SignalingHeme TransportPlesiomonas ShigelloidesHeme HomeostasisRecombinant Hemoglobin ProductionBiomolecular EngineeringBiomanufacturingNatural SciencesHeme DegradationBiotechnologySynthetic BiologyProtein EngineeringMicrobiology
To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.
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