Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

Ryan Choi, Angela Kelley, D.J. Leibly, +3

Acta Crystallographica Section F Structural Biology and Crystallization Communications

Crystal StructureCrystallographic Structure SuccessMolecular BiologyEscherichia ColiProtein Purification +19

High-throughput protein production and purification at the Seattle Structural Genomics Center for Infectious Disease

Cassie M. Bryan, Janhavi Bhandari, Alberto J. Napuli, +6

Acta Crystallographica Section F Structural Biology and Crystallization Communications

Structural BioinformaticsMolecular BiologyInfectious DiseaseHigh-throughput Protein ProductionSsgcid Proteins +15

Selective Inhibitors of Methionyl-tRNA Synthetase Have Potent Activity against Trypanosoma brucei Infection in Mice

S. Shibata, J. Robert Gillespie, Angela Kelley, +12

Antimicrobial Agents and Chemotherapy

Toxoplasma gondii Cathepsin L Is the Primary Target of the Invasion-inhibitory Compound Morpholinurea-leucyl-homophenyl-vinyl Sulfone Phenyl

E.T. Larson, Fabiola Parussini, My‐Hang Huynh, +6

Journal of Biological Chemistry

Protein SecretionAntiparasitic AgentPathologyMolecular BiologyCytoskeleton +16

Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate

E.T. Larson, Jessica E. Kim, Frank Zucker, +9

Biochimie

BiosynthesisCellular EnzymologyBiochemistryNatural SciencesBiocatalysis +7

Crystal Structures of Trypanosomal Histidyl-tRNA Synthetase Illuminate Differences between Eukaryotic and Prokaryotic Homologs

E.A. Merritt, T.L. Arakaki, J. Robert Gillespie, +12

Journal of Molecular Biology

Prokaryotic SystemBiochemistryMedicineNatural SciencesProtein X-ray Crystallography +6

Enhancement of Recombinant Hemoglobin Production in <i>Escherichia coli</i> BL21(DE3) Containing the <i>Plesiomonas shigelloides</i> Heme Transport System

D. Villarreal, Cynthia L. Phillips, Angela Kelley, +5

Applied and Environmental Microbiology

EngineeringMolecular BiologyEscherichia ColiTransport GenesAnalytical Ultracentrifugation +16

Structure of a Burkholderia pseudomallei Trimeric Autotransporter Adhesin Head

Thomas E. Edwards, Isabelle Phan, Jan Abendroth, +12

PLoS ONE

Expression of proteins in<i>Escherichia coli</i>as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline

Stephen N. Hewitt, Ryan Choi, Angela Kelley, +3

Acta Crystallographica Section F Structural Biology and Crystallization Communications

Molecular BiologyEscherichia ColiAnalytical UltracentrifugationProtein SynthesisProtein Purification +14

X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source

Jan Abendroth, Michael S. McCormick, Thomas E. Edwards, +15

Journal of Structural and Functional Genomics

X-ray CrystallographyX-ray Structure DeterminationBiochemistryNatural SciencesMycobacterium Tuberculosis +5