FEMS Microbiology Reviews · 1994 · 12 citations · 7 references
Life SciencesBiotechnologyMicrobiologyBiochemical EngineeringBioprocess EngineeringFermentation EngineeringBiofuel ProductionBioreactor OperationContinuous Bioreactor OperationContinuous FermentationFermentation KineticsFluidized-bed BioreactorsContinuous Ethanol FermentationEthanol FermentationEthanol ProductionFluidized-bed FermentationHigh-cell-density FermentationMicrobial Ethanol ProductionSaccharomyces Cerevisiae StabilityYeast Fermentation
Continuous fermentation by a highly flocculant strain of the yeast Saccharomyces cerevisiae was carried out in a tower fluidized-bed bioreactor. The synthetic and molasses media with a total sugar concentration of 17% (w/v) were used for fermentation. Different dilution rates were tested. Stable cell densities of 50 kg m-3 (dry weight) were maintained for all dilution rates. The ethanol productivity was increasing linearly with dilution rates up to 15-20 kg m-3 h-1. Aeration of the culture stabilized flocculating activity and viability of yeast and also permitted long-term operation of the bioreactor.
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