Free-radical scavenging activity and phytochemical analysis in the leaf and stem of Drymaria diandra Blume.

Abstract

In different parts of plain land and hill area of Darjeeling district, leaves and stems of Drymaria diandra were evaluated for their phytochemical constituents like total phenol, ortho-dihydric phenol, flavonols, tannins and antioxidant activity against 2,2-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, nitric oxide radical and anti-lipid peroxidation activity. Strong antioxidant scavenging activities were observed in both leaf and stem in different places. Anti-oxidative efficiency to inhibit anti-lipid peroxidation of these plant extracts in goat liver was investigated. Drymaria diandra stem showed moderate class of anti-lipid peroxidation against thioburbituric acid but the leaves have high anti-lipid peroxidation activity. The correlation was also drawn with antioxidants, its attributes and soil nutrients profile. This plant extract may be explored as therapeutic agent in future.