Increasing Methotrexate Resistance by Combination of Active-site Mutations in Human Dihydrofolate Reductase

Prediction of protein conformation

Peter Y. Chou, Gerald D. Fasman · Biochemistry · 1974 · 3.3K citations

Life Sciences, Biochemistry, Bioinformatics +9

Enzyme kinetics; behavior and analysis of rapid equilibrium and steady state enzyme systems

International Journal of Biochemistry · 1994 · 3K citations

Life Sciences, Biochemistry, Chemistry +16

The "megaprimer" method of site-directed mutagenesis.

Gobinda Sarkar, S S Sommer · PubMed · 1990 · 1.2K citations

Life Sciences, Genetics, Molecular Biology +12

The nature of the accessible and buried surfaces in proteins

Cyrus Chothia · Journal of Molecular Biology · 1976 · 1.2K citations

Life Sciences, Biochemistry, Biophysics +12

The 'evolvability' of promiscuous protein functions

Amir Aharoni, Leonid Gaidukov, Olga Khersonsky et al. · Nature Genetics · 2004 · 832 citations

Life Sciences, Biochemistry, Molecular Biology +12