Azido‐BODIPY Acid Reveals Quantitative Staudinger–Bertozzi Ligation in Two‐Step Activity‐Based Proteasome Profiling

Abstract

Flexibility in protein profiling: Employing the new bifunctional fluorophore azido-BODIPY acid we have synthesized a compatible set of one-step and two-step proteasome probes and demonstrate that with these the efficiency of the Staudinger–Bertozzi ligation in the two-step activity-based protein profiling of the proteasome catalytic activities is easily established. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2008/z800231_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.