In Vivo Insertional Mutagenesis in <i>Corynebacterium pseudotuberculosis</i> : an Efficient Means To Identify DNA Sequences Encoding Exported Proteins

Abstract

The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown-function proteins.