A Biosynthetic Route to Dehydroalanine‐Containing Proteins

Abstract

It's not natural: The nonnatural amino acid phenylselenocysteine has been genetically incorporated into proteins in E. coli in response to the amber nonsense codon TAG. Oxidative elimination followed by Michael addition led to incorporation of (S)-hexadecyl- and (S)-mannosylcysteine into green fluorescent protein. This approach provides a useful method to modify protein side chains and backbone structures. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z702305_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.