A note on the isolation of salmonellas from environmental samples using three formulations of Rappaport's broth

Abstract

The efficiency of isolation of salmonellas from 412 seagull intestines and 247 polluted water samples was compared using three formulations of Rappaport's medium (RV, RV‐soya and R25). A modification of RV medium (RV‐soya) was shown to be the most efficient of the three media and it was shown that the duration of incubation of this medium could be restricted to 24 h. Inoculation ratios were compared for RV and RV‐soya and 1:100 was shown to be significantly better than 1:20. It is concluded that RV‐soya is at least as efficient as the standard RV medium and its use can therefore be recommended.