Binding of Taurocholate by Pectin in the Presence of Calcium Ions

Abstract

ABSTRACT Binding of taurocholate by pectin was examined in vitro , using low‐methoxyl pectin and calcium lactate. Water in calcium pectate gel was partially separated from the gel by filtration through a glass‐filter. Taurocholate bound by pectin was calculated after measurement of taurocholate in the filtrate by the UV absorption method. The amount of taurocholate bound by pectin increased linearly with increase in pectin concentration but independent of time. One gram pectin bound a maximum of 6.0 μmole of taurocholate.