Journal of Virology · 1998 · 101 citations · 29 references
Life SciencesMolecular BiologyMolecular GeneticsMolecular VirologyPlant VirologyProtein TranslationProtein SynthesisTranslation InitiationTranslation RegulationTranslational RegulationViral Rna BiologyVirus Genome OrganizationMessenger Ribonucleic Acid (Mrna) TranslationMrna Translation InitiationOverlapping Open Reading FramesRibosomal Leaky ScanningRibosome ScanningSubgenomic Rna SynthesisViral Rna TranslationViral Translation Mechanisms
The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.
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Site-directed mutagenesis by overlap extension using the polymerase chain reaction
Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations
Eukaryotic start and stop translation sites
Douglas R. Cavener, Stuart C. Ray · Nucleic Acids Research · 1991 · 593 citations · Full text