Mass spectrometric identification of triacylglycerols of enzymatically modified butterfat separated on a polarizable phenylmethylsilicone column

Abstract

Abstract Original butter oil, butter oil interesterified with Pseudomonas fluorescens lipase as catalyst, and the saturated, monoene, diene and triene fractions of interesterified butter oil from silver ion chromatography on a p ‐propylbenzene sulfonic acid column were analyzed on a polarizable phenyl(65%)methylsilicone column. Major and also some minor molecular species of triacylglycerols (TG) were identified from the electron‐impact (EI) gas chromatography/mass spectrometry analytical data for the original and lipase‐modified butter oil and the four fractions. Acyl + 74 and acyl + 128 fragments of EI mass spectral data proved useful, in addition to acyl and M ‐ RCOO fragments, in the identification of molecular species of TG. The TG compositions of the modified butter oils were compared with the composition calculated according to random distribution and with the TG composition of original butter oil.