FEMS Microbiology Letters · 2003 · 77 citations · 24 references
MedicineBiotechnologyMicrobiologyCancer ImmunologyImmunizationVaccinologyCancer VaccinesCervical CancerHuman Papillomavirus InfectionLactic Acid BacteriaOncogenic VirusesVaccinationCell Surface DisplayCell Surface GlycoproteinsE7 ExpressionE7 OncoproteinImmune Response To HpvIntranasal AdministrationTherapeutic Hpv VaccinesTherapeutic Human Papillomavirus Vaccines
E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.
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Treatment of Murine Colitis by <i>Lactococcus lactis</i> Secreting Interleukin-10
Lothar Steidler, Wolfgang Hans, L. Schotte et al. · Science · 2000 · 1.5K citations