Proceedings of the National Academy of Sciences · 1969 · 98 citations · 14 references
Life SciencesBiochemistryMolecular BiologyProtein BiochemistryProtein TranslationRna ProcessingCodon RecognitionProtein SynthesisRibosome FunctionTranslation FactorsTranslation TerminationTranslational RegulationAmmonium Sulfate FractionationBacterial Translation TerminationChain TerminationNascent Peptide ReleasePurificationRelease Factor FunctionStop Codon RecognitionTranslation Termination Factors
Throughout extensive purification, the release factors R(1) and R(2) each behave as a single molecular species with alternate codon recognition (R(1), UAA or UAG; R(2), UAA or UGA). The release of f[(3)H]methionine from f[(3)H]-Met-tRNA.AUG.ribosome complex requires R factor and terminator codon and does not appear to require tRNA or transfer factors T and G. Purification of the components of the release assay has enabled identification of a protein factor S in the 55-80 per cent ammonium sulfate fraction of E. coli B supernatant fraction which stimulates the rate but not the extent of release dependent upon R factor and appropriate termination codon. The S factor has properties similar to T, but further purification is required to determine the nature and function of S in peptide chain termination.
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Procedures in Nucleic Acid Research.
Waldo E. Cohn · Journal of the American Chemical Society · 1967 · 2K citations
RNA Codewords and Protein Synthesis
Marshall W. Nirenberg, Philip Leder · Science · 1964 · 1.4K citations