Veterinary Drug Residues Survey in Meat:  An HPLC Method with a Matrix Solid Phase Dispersion Extraction

Abstract

A multiresidue technique for the isolation and liquid chromatography determination of 14 veterinary drug residues in meat is described (10 antibiotics, 2 anthelmintics, 1 coccidiostat, and 1 other chemotherapeutic drug). Matrix solid phase dispersion (MSPD) was chosen as the extraction technique for this screening method. Two fractions were collected by elution with methylene chloride and ethyl acetate. No additional purification was needed. A reversed-phase C18 column was used for the high performance liquid chromatography (HPLC) to separate the analytes with gradients of ammonium acetate buffer−acetonitrile−methanol as mobile phase. Photodiode array and fluorimetric detectors were used for this analysis. Validation data are presented. The limits of detection for each analyte are shown and are under the regulatory tolerances. The percentage recoveries and the linearity of the method were evaluated comparing spiked samples and standard solutions. Meat naturally contaminated with sulfamethazine was used for the evaluation of the repeatability of the method, which was satisfactory. Keywords: Matrix solid phase dispersion; HPLC; sulfonamides; benzimidazoles; nicarbazin; chloramphenicol; furazolidone